Specifications | The use of luminous bacteria for the assessment of air quality \Focus on IFA s work No. 0220\ IFA |
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Specifications | The use of luminous bacteria for the assessment of air quality \Focus on IFA s work No. 0220\ IFA |
Business section |
Specifications | The use of luminous bacteria for the assessment of air quality \Focus on IFA s work No. 0220\ IFA |
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Content | 0220 Edition 7/2012 617.0-IFA:638.1 The use of luminous bacteria for the assessment of air quality Problem An important function of occupational health and safety is the identification, assessment and monitoring of hazardous substances in workplace atmospheres. This function is normally fulfilled by chemical analysis of a substance or selected number of substances for the monitoring of atmospheric limit values. Due to its complexity and unknown interactions assessment of the total toxicity of the substance mixture actually present is seldom possible by this means. Activities A standardised environmental toxicity test devel- oped for the monitoring of water quality was adapted to the specific requirements of assess- ment of workplace atmosphere samples. Sampling can be performed in the usual way by means of air sampling adsorbents and the toxicity determined in a central laboratory. Bacteria of marine origin with the characteristic of bioluminescence (“cold light”) are employed as biological sensors. Contact with toxic substances results in an inhibition of light emission, which in turn can be quantified by suitable instruments and constitutes a measure of the total toxicity of a test substance. Results and Application The measurement method has proved effective in laboratory-scale testing involving defined test Luminous bacteria on a Petri disk gases (discrete substances) in the order of mag- nitude of the maximum workplace concentrations. The method was employed routinely in test-cham- ber experiments for the study of emissions from colour laser printers and colour photocopiers under realistic conditions. Trials were also per- formed successfully during the hot application of bitumen in field tests at real-case workplaces. In the case of lipophilic (fat-soluble) substances the luminous bacteria test appears to be of some- what restricted efficacy. It responds with dispro- portionate sensitivity to this class of substances, which limits the scope for its use. |
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